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The ribose rings having vic-cis-diol groups were located inside the active sites, where the phosphodiester substrates were also accommodated (Scheme 15). 30 Man Jung Han, Ji Young Chang The vic-cis-diol groups formed hydrogen bonds with the two phosphoryl oxygen atoms of the substrate so as to activate the phosphorus atoms to be attacked by nucleophiles (H2O) and the alkoxy groups to be left either by an inline or by an adjacent mechanism. In the biological system there are plenty of biopolymers containing riboses with free vic-cis-diol, which might show nuclease activities.

Peppas and Merrill [9, 12] examined the phenomenon of partial crystallization by a process of dehydration and annealing. Resulting materials contained crystallites in addition to crosslinks. The crystalline regions essentially served as additional crosslinks to redistribute external stresses. The degree of crystallinity of samples annealed at 120  C for 30 min is shown as a function of the crosslinking density in Fig. 3. A third mechanism of hydrogel preparation involves ªphysicalº crosslinking due to crystallite formation [13].

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A 10-point circle is associated with any general point of the ellipse. New properties of Fagnanos point by Ternullo M.


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