By Weilie Zhou
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Extra info for Advanced scanning microscopy for nanotechnology techniques and applications
Serves as a mordant that interacts with itself and with other stains. The use of mordants for high-resolution SEM is not recommended because it binds additional elements to membranes such that at structural resolution of 1–10 nm the observed structures are artificially thickened and the features dimensions not accurately recorded. Mordant methods have been very elegantly employed for intermediate magnification of cellular organelles . 3. Dehydration and Air drying Subsequent to fixation, the aqueous content of the sample is replaced with an intermediate fluid, usually an organic solvent such as ethanol or acetone before drying.
Oatley, The Scanning Electron Microscope, Cambridge University Press, Cambridge (1972). 7. J. I. Goldstein and H. Yakowitz, Practical Scanning Electron Microscopy, Plenum Press, New York (1975). 40 Weilie Zhou et al. 8. Y. X. Chen, L. J. Campbell, and W. L. Zhou, J. Cryst. Growth, 270 (2004) 505. 9. J. J. Liu, A. West, J. J. Chen, M. H. Yu, and W. L. Zhou, Appl. Phys. , 87 (2005) 172505. 10. T. E. Everhart and R. F. Thornley, J. Sci. , 37 (1960) 246. 11. D. C. Joy, C. S. Joy, and R. D. Bunn, Scanning, 18 (1996) 533.
On the one hand, if the molecules were incident from the backing pump side to the chamber side, it will be pushed to the backing pump side. In this way, a preferred gas flow direction is created and a pressure difference is maintained across vane disk. A typical turbo pump contains several rotating disks with the vane angle decreasing at each stage. The pumping speed of the turbo pump decreases rapidly at pressure above a certain level (near 10−3 Torr). 32 Weilie Zhou et al. 27. Schematic of a pumping stage in turbo pump.
Advanced scanning microscopy for nanotechnology techniques and applications by Weilie Zhou